Development and optimization of Andrographis paniculata extract-loaded phytosomes using Box-Behnken design approach

Abstract

In current research, Andrographis paniculata extracts loaded phytosomes were successfully synthesized by thin-film hydration technique using soy lecithin and cholesterol and optimized by Box-Behnken design. The quadratic equation for % entrapment efficiency was found Y1 = 69.22 + 15.23 X1 –  0.7775 X2 – 2.65 X3 – 1.025 X1X2 + 2.41 X1X3 – 1.56 X2X3 + 6.95 X1² – 7.21 X2² – 5.52 X3² and for percentage yield was found Y2 = 70.43 + 13.86 X1 – 0.2025 X2 – 0.6962 X3 – 0.2425 X1X2 + 1.86 X1X3 – 1.43 X2X3 + 6.47 X1² – 6.75 X2² – 3.83 X3². These equations demonstrated that lipid: drug (X1) have significant effect on entrapment efficiency (Y1) and yield (Y2). The values of independent variables for optimized phytosomes were lipid: extract (X1 = 1: 1 w/w), temperature (X2 = 43^oC) and time (X3 = 2.15 hours) which have D-value of 0.929. The % bias between actual and predicted values of Y1 and Y2 was 1.25% and 2.36%, respectively which concluded authenticity of design model.

URN:NBN:sciencein.jist.2023.v11.558