In-vitro antioxidant analysis of Aristolochia indica, Ipomoea obscura, Tylophora indica, Glinus oppositifolius and Abroma augustum from Bankura district, West Bengal

Abstract

Five therapeutic plants that have been utilized traditionally across the Sonamukhi Block of Bankura District, West Bengal, were tested for antioxidant activity using three assays: ABTS radical scavenging activity, FRAP reduction power, and DPPH free radical scavenging. According to the DPPH assay, Glinus oppositifolius (68.4%) and Ipomoea obscura (23.83%) showed moderate radical-scavenging activity, whereas Aristolochia indica (73.07%), Abroma augustum (52.87%), and Tylophora indica (25%) demonstrated the highest levels. While Glinus oppositifolius (0.685) and Ipomoea obscura (0.401) showed moderate activity in the FRAP assay, Abroma augustum (0.459), Tylophora indica (0.637), and Aristolochia indica (0.545) demonstrated significant reducing power. According to the ABTS assay, Aristolochia indica (90.37%) and Glinus oppositifolius (98.7%) had the highest levels of radical scavenging activity.  These findings support the traditional medical usage of these plants, especially Glinus oppositifolius and Aristolochia indica, which showed the most antioxidant qualities. The results highlight the importance of these plants in traditional medicine, shed light on their therapeutic potential, and lay the groundwork for further research on natural antioxidant treatments.