Synthesis of new regioselective imidazole-isoxazole hybrids as in vitro antibacterial agents

The one-pot synthesis of some novel imidazole-isoxazole hybrids (5a-5n) and their in vitro antibacterial evaluation against Bacillus subtilis and Staphylococcus aureus was described herein. Among all, compounds 5f, 5g, 5i and 5j displayed greater zone of inhibition on Staphylococcus aureus than the standard streptomycin. Besides, compounds 5f, 5g, 5h, 5i, 5j and 5k displayed higher zone of inhibition towards Bacillus subtilis than the standard. Lastly, minimum inhibitory concentration (MIC) studies of active compounds 5f, 5g, 5h, 5i, 5j and 5k showed that the compounds 5i and 5j having lesser MIC values on Staphylococcus aureus and Bacillus subtilis than the standard.