Phytochemical and in vitro analysis of Bornetella oligospora extract as alpha-Glucosidase inhibitor

Abstract

One of the first-line therapy of diabetes mellitus is alpha-glucosidase inhibitors. Due to side effects caused by synthetic drugs and limited sources, various studies have been conducted on herbal plants and macroalgae consisting of bioactive compounds with alpha-glucosidase inhibitory activity. This study examines the alpha-glucosidase inhibitory activity and phytochemical compounds in ethyl acetate and ethanol extract of Bornetella oligospora, an abundant green alga in eastern Indonesia. The result showed that ethanol and ethyl acetate extract of Bornetella oligospora contained phytochemical components such as flavonoid, glycoside, triterpenoid, and steroid. The thin layer chromatography test showed ethanol extract have five spots with Rf 0.545, 0.527, 0.5, 0.473, and 0.154, while the ethyl acetate extract has two spots with Rf 0.58 and 0.64. The alpha-glucosidase inhibition assay showed IC50 values of the ethanol extract was 11.702 ug/mL and ethyl acetate extract was 95.384 ug/mL. In conclusion, Bornetella oligospora extract has the potential as an antidiabetic agent.